Journal article
Urinary estrone conjugate and pregnanediol 3-glucuronide enzyme immunoassays for population research
Clinical Chemistry, Vol.49(7), pp.1139-1148
Jul/2003
Abstract
Background. Monitoring of reproductive steroid hormones at the population level requires frequent measurements, hormones or metabolites that remain stable under less than ideal collection and storage conditions, a long-term supply of antibodies, and assays useful for a range of populations. We developed enzyme immunoassays for urinary pregnanediol 3-glucuronide (PDG) and estrone conjugates (E1Cs) that meet these criteria. Methods: Enzyme immunoassays based on monoclonal antibodies were evaluated for specificity, detection limit, parallelism, recovery, and imprecision. Paired urine and serum specimens were analyzed throughout menstrual cycles of 30 US women. Assay application in different populations was examined with 23 US and 42 Bangladeshi specimens. Metabolite stability in urine was evaluated for 0-8 days at room temperature and for 0-10 freeze-thaw cycles. Results: Recoveries were 108% for the PDG assay and 105% for the E1C assay. Serially diluted specimens exhibited parallelism with calibration curves in both assays. Inter- and intraassay CVs were,
Details
- Title
- Urinary estrone conjugate and pregnanediol 3-glucuronide enzyme immunoassays for population research
- Creators
- KA O'Connor (null)E Brindle (null)DJ Holman (null)NA Klein (null)MR Soules (null)KL Campbell (null)F Kohen (null)CJ Munro (null)JB Shofer (null)BL Lasley (null)JW Wood (null)
- Resource Type
- Journal article
- Publication Details
- Clinical Chemistry, Vol.49(7), pp.1139-1148; Jul/2003
- Number of pages
- 10
- Language
- English
- DOI
- https://doi.org/10.1373/49.7.1139
- Scientific Unit
- The Weizmann Institute of Science
- Record Identifier
- 993267346103596
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