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Preprint (Author's original)CC BY-NC-ND V4.0, Open Access
Journal article
The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans
Science (American Association for the Advancement of Science), Vol.376(6594), eabl4896
13/May/2022
PMID: 35549404
Abstract
Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type-specific RNA splicing was discovered and analyzed across tissues within an individual.
Details
- Title
- The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans
- Creators
- Robert C JonesJim KarkaniasMark A KrasnowAngela Oliveira PiscoStephen R Quake - Chan Zuckerberg InitiativeJulia SalzmanNir Yosef - University of California, BerkeleyBryan BulthaupPhillip BrownWilliam HarperMarisa HemenezRavikumar PonnusamyAhmad SalehiBhavani A SanagavarapuEileen SpallinoKsenia A AaronWaldo ConcepcionJames M Gardner - University of California, San FranciscoBurnett KellyNikole NeidlingerZifa WangSheela CrastaSaroja KolluruMaurizio MorriSerena Y TanKyle J TravagliniChenling XuMarcela Alcántara-HernándezNicole AlmanzarJane AntonyBenjamin BeyersdorfDeviana BurhanKruti CalcuttawalaMatthew M CarterCharles K F ChanCharles A ChangStephen ChangAlex ColvilleRebecca N CulverIvana CvijovićGaetano D'AmatoCamille EzranFrancisco X GaldosAstrid GillichWilliam R GoodyerYan HangAlyssa HayashiSahar HoushdaranXianxi HuangJuan C IrwinSoRi JangJulia Vallve JuanicoAaron M KershnerSoochi KimBernhard KissWilliam KongMaya E KumarAngera H KuoRebecca LeylekBaoxiang LiGabriel B LoebWan-Jin LuSruthi MantriMaxim Markovic - Stanford UniversityPatrick L McAlpineAntoine de MorreeKarim MroujShravani MukherjeeTyler MuserPatrick NeuhöferThi D NguyenKimberly PerezRagini PhansalkarNazan PulucaZhen QiPoorvi RaoHayley Raquer-McKayNicholas SchaumBronwyn ScottBobak SeddighzadehJoe SegalSushmita SenShaheen SikandarSean P Spencer - University of PennsylvaniaLea C SteffesVarun R SubramaniamAditi SwarupMichael SwiftWill Van Treuren - Stanford UniversityEmily TrimmStefan VeizadesSivakamasundari VijayakumarKim Chi VoSevahn K VorperianWanxin WangHannah N W WeinsteinJuliane WinklerTimothy T H WuJamie XieAndrea R YungYue ZhangAngela M DetweilerHoney MekonenNorma F NeffRene V SitMichelle TanJia YanGregory R BeanVivek CharuErna Forgó - Stanford University School of MedicineBrock A MartinMichael G OzawaOscar SilvaAngus TolandVenkata N P VemuriShaked AfikKyle AwayanOlga Borisovna BotvinnikAshley ByrneMichelle ChenRoozbeh DehghannasiriAdam Gayoso - University of California, BerkeleyAlejandro A GranadosQiqing LiGita MahmoudabadiAaron McGeeverJulia Eve OlivieriMadeline ParkNeha RavikumarGeoff StanleyWeilun TanAlexander J TarashanskyRohan VanheusdenPeter WangSheng Wang - Peking UniversityGalen Xing - University of California, BerkeleyRebecca CulverLes DethlefsenPo-Yi Ho - Harvard UniversityShixuan LiuJonathan S MaltzmanRoss J MetzgerKoki SasagawaRahul SinhaHanbing SongBruce WangSteven E ArtandiPhilip A Beachy - Stanford UniversityMichael F ClarkeLinda C GiudiceFranklin W HuangKerwyn Casey HuangJuliana IdoyagaSeung K KimMark KrasnowChristin S KuoPatricia NguyenThomas A RandoKristy Red-Horse - Stanford UniversityJeremy ReiterDavid A RelmanJustin L Sonnenburg - Stanford UniversityAlbert Wu - Northwestern UniversitySean M WuTony Wyss-Coray - Stanford University School of MedicineTabula Sapiens Consortium
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.376(6594), eabl4896; 13/May/2022
- Number of pages
- 11
- Language
- English
- DOI
- https://doi.org/10.1126/science.abl4896
- PMID
- 35549404
- Grant note
- We express our gratitude and thanks to donor WEM and his family, as well as to all the anonymous organ and tissue donors and their families for giving both the gift of life and the gift of knowledge through their generous donations. We also thank Donor Network West for their partnership on this project, the UCSF Liver Center (funded by NIH P30DK026743) for assistance with the liver cell isolations, S. Schmid for a close reading of the manuscript, and B. Tojo for the original artwork in Fig. 1. The data portal for this publication is available at (14).Funding: This project has been made possible in part by grant nos. 2019-203354,2020-224249, and 2021-237288 from the Chan Zuckerberg Initiative DAF; an advised fund of Silicon Valley Community Foundation; and by support from the Chan Zuckerberg Biohub.
- Record Identifier
- 993420483403596
Metrics
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