Journal article
Single-cell epigenomics: techniques and emerging applications
Nature Reviews Genetics, Vol.16(12), pp.716-726
01/Dec/2015
Abstract
Epigenomics is the study of the physical modifications, associations and conformations of genomic DNA sequences, with the aim of linking these with epigenetic memory, cellular identity and tissue-specific functions. While current techniques in the field are characterizing the average epigenomic features across large cell ensembles, the increasing interest in the epigenetics within complex and heterogeneous tissues is driving the development of single-cell epigenomics. We review emerging single-cell methods for capturing DNA methylation, chromatin accessibility, histone modifications, chromosome conformation and replication dynamics. Together, these techniques are rapidly becoming a powerful tool in studies of cellular plasticity and diversity, as seen in stem cells and cancer.
Details
- Title
- Single-cell epigenomics; techniques and emerging applications
- Creators
- Omer Schwartzman (null)Amos Tanay (null) - 972WIS_INST___83
- Resource Type
- Journal article
- Publication Details
- Nature Reviews Genetics, Vol.16(12), pp.716-726; 01/Dec/2015
- Number of pages
- 11
- Language
- English
- DOI
- https://doi.org/10.1038/nrg3980
- Grant note
- Research in the A.T. group is supported by the European Research Council, the Israel Science Foundation and the Flight Attendant Medical Research Institute. Work by O.S. was done as part of the requirements for Ph.D. theses at Tel Aviv University.
- Record Identifier
- 993267752803596
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