Journal article
Paradoxical Role of DNA Methylation in Activation of FoxA2 Gene Expression during Endoderm Development
Journal of Biological Chemistry, Vol.289(34), pp.23882-23892
Aug/2014
Abstract
Background: The transcription factor FoxA2 is a key mediator of endoderm development and pancreas gene expression. Results: DNA methylation is paradoxically associated with active expression of the FoxA2 gene. Conclusion: Developmental control genes such as FoxA2 may be regulated by a novel genetic mechanism. Significance: Clarification of this mechanism may provide a better understanding of how developmental pathways are activated. The transcription factor FoxA2 is a master regulator of endoderm development and pancreatic beta cell gene expression. To elucidate the mechanisms underlying the activation of the FoxA2 gene during differentiation, we have compared the epigenetic status of undifferentiated human embryonic stem cells (hESCs), hESC-derived early endoderm stage cells (CXCR4+ cells), and pancreatic islet cells. Unexpectedly, a CpG island in the promoter region of the FoxA2 gene displayed paradoxically high levels of DNA methylation in expressing tissues (CXCR4+, islets) and low levels in nonexpressing tissues. This CpG island region was found to repress reporter gene expression and bind the Polycomb group protein SUZ12 and the DNA methyltransferase (DNMT)3b preferentially in undifferentiated hESCs as compared with CXCR4+ or islets cells. Consistent with this, activation of FoxA2 gene expression, but not CXCR4 or SOX17, was strongly inhibited by 5-aza-2-deoxycytidine and by knockdown of DNMT3b. We hypothesize that in nonexpressing tissues, the lack of DNA methylation allows the binding of DNA methyltransferases and repressing proteins, such as Polycomb group proteins; upon differentiation, DNMT activation leads to CpG island methylation, causing loss of repressor protein binding. These results suggest a novel and unexpected role for DNA methylation in the activation of FoxA2 gene expression during differentiation.
Details
- Title
- Paradoxical Role of DNA Methylation in Activation of FoxA2 Gene Expression during Endoderm Development
- Creators
- Keren Bahar Halpern (null)Tal Vana (null) - 972WIS_INST___112Michael Walker (null) - 972WIS_INST___112
- Resource Type
- Journal article
- Publication Details
- Journal of Biological Chemistry, Vol.289(34), pp.23882-23892; Aug/2014
- Number of pages
- 11
- Language
- English
- DOI
- https://doi.org/10.1074/jbc.M114.573469
- Grant note
- Juvenile Diabetes Research Foundation; Israel Science Foundation; Helen and Martin Kimmel Institute for Stem Cell Research at the Weizmann InstituteThis work was supported by grants from the Juvenile Diabetes Research Foundation, the Israel Science Foundation, and the Helen and Martin Kimmel Institute for Stem Cell Research at the Weizmann Institute (to M. D. W.)._ALMAME_DELIMITER_
- Record Identifier
- 993265991903596
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