Journal article
PROSS 2: a new server for the design of stable and highly expressed protein variants
Bioinformatics (Oxford, England), Vol.37(1), pp.123-125
26/Dec/2020
PMID: 33367682
Abstract
Many natural and designed proteins are only marginally stable limiting their usefulness in research and applications. Recently, we described an automated structure and sequence-based design method, called PROSS, for optimizing protein stability and heterologous expression levels that has since been validated on dozens of proteins. Here, we introduce improvements to the method, workflow and presentation, including more accurate sequence analysis, error handling and automated analysis of the quality of the sequence alignment that is used in design calculations. PROSS2 is freely available for academic use at https://pross.weizmann.ac.il.
Details
- Title
- PROSS 2; a new server for the design of stable and highly expressed protein variants
- Creators
- Jonathan Jacob Weinstein - 972WIS_INST___112Adi Goldenzweig - 972WIS_INST___112Shlomo-Yakir Hoch - 972WIS_INST___112Sarel Jacob Fleishman - 972WIS_INST___112
- Resource Type
- Journal article
- Publication Details
- Bioinformatics (Oxford, England), Vol.37(1), pp.123-125; 26/Dec/2020
- Number of pages
- 3
- Language
- English
- DOI
- https://doi.org/10.1093/bioinformatics/btaa1071
- PMID
- 33367682
- Grant note
- We thank users from around the world for their feedback and support of the PROSS web server. We also thank Shiran Barber-Zucker for valuable feedback on the new server, Ziv Avizemer, Dina Listov and Olga Khersonsky for testing PROSS2, and Jaime Prilusky and Rotem Barzilay for excellent technical support. This research was funded by the European Research Council [815379]; the Israel Science Foundation [1844]; the Israel Ministry of Science [128625]; the Milner Foundation; and a charitable donation from Sam Switzer and family.
- Record Identifier
- 993278604603596
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