Journal article
NMR structure and binding of esculentin-1a (1-21)NH2 and its diastereomer to lipopolysaccharide: Correlation with biological functions
Biochimica Et Biophysica Acta-Biomembranes, Vol.1858(4), pp.800-812
Apr/2016
Abstract
The frog skin-derived antimicrobial peptide esculentin-1a(1-21)NH2 [Esc(1-21)], and its diastereomer Esc(1-21)-1c (containing two D-amino acids at positions 14 and 17), have been recently found to neutralize the toxic effect of Pseudomonas aeruginosa lipopolysaccharide (LPS), although to different extents. Here, we studied the three-dimensional structure of both peptides in complex with P. aeruginosa LPS, by transferred nuclear Overhauser effect spectroscopy. Lack of NOE peaks revealed that both the peptides adopted a random coil structure in aqueous solution. However, Esc(1-21) adopted an amphipathic helical conformation in LPS micelles with 5 basic Lys residues forming a hydrophilic cluster. In comparison, the diastereomer maintained an alpha helical conformation only at the N-terminal region, whereas the C-terminal portion was quite flexible. Isothermal titration calorimetry (ITC) revealed that the interaction of Esc(1-21) with LPS is an exothermic process associated with a dissociation constant of similar to 4 mu M. In contrast, Esc(1-21)-1c had almost 8 times weaker binding affinity to LPS micelles. Moreover, STD NMR data supported by docking analysis have identified those amino acid residues responsible for the peptide's binding to LPS micelles. Overall, the data provide important mechanistic insights on the interaction of esculentin-derived peptides with LPS and the reason for their different anti-endotoxin activity. These data might also assist to further design more potent antimicrobial peptides with antisepsis properties, which are highly needed to overcome the widespread concem of the available anti-infective agents. (C) 2015 Elsevier B.V. All rights reserved.
Details
- Title
- NMR structure and binding of esculentin-1a (1-21)NH2 and its diastereomer to lipopolysaccharide; Correlation with biological functions
- Creators
- Antina Ghosh (null) - Bose InstituteS Bera (null) - Bose InstituteYechiel Shai (null) - 972WIS_INST___112ML Mangoni (null) - Sapienza University of RomeA Bhunia (Corresponding Author) - Bose Institute
- Resource Type
- Journal article
- Publication Details
- Biochimica Et Biophysica Acta-Biomembranes, Vol.1858(4), pp.800-812; Apr/2016
- Number of pages
- 13
- Language
- English
- DOI
- https://doi.org/10.1016/j.bbamem.2015.12.027
- Grant note
- Sapienza University di Roma; Italian Foundation for Cystic Fibrosis [FFC#11/2014]; DST FAST track Govt. of India [SR/FT/LS-100/2012]; CSIR; UGC. This work was supported by grants from Sapienza Università di Roma and the Italian Foundation for Cystic Fibrosis (Project FFC#11/2014 adopted by FFC Delegations from Siena, Sondrio Valchiavenna, Cerea Il Sorriso di Jenny and Pavia). Part of this work are object of a pending US patent application no. 14/506,383. AB thanks DST FAST track (SR/FT/LS-100/2012) Govt. of India for financial support. AG and SB thank CSIR and UGC, respectively for PhD fellowship. Central instrument facility (CIF) of Bose Institute and DBT-CU, IPLS facility are greatly acknowledged for NMR and ITC experiments, respectively._ALMAME_DELIMITER_
- Record Identifier
- 993268086303596
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