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Journal article
Modern venomics-Current insights, novel methods, and future perspectives in biological and applied animal venom research
Gigascience, Vol.11, giac048
18/May/2022
PMID: 35640874
Abstract
Venoms have evolved >100 times in all major animal groups, and their components, known as toxins, have been fine-tuned over millions of years into highly effective biochemical weapons. There are many outstanding questions on the evolution of toxin arsenals, such as how venom genes originate, how venom contributes to the fitness of venomous species, and which modifications at the genomic, transcriptomic, and protein level drive their evolution. These questions have received particularly little attention outside of snakes, cone snails, spiders, and scorpions. Venom compounds have further become a source of inspiration for translational research using their diverse bioactivities for various applications. We highlight here recent advances and new strategies in modern venomics and discuss how recent technological innovations and multi-omic methods dramatically improve research on venomous animals. The study of genomes and their modifications through CRISPR and knockdown technologies will increase our understanding of how toxins evolve and which functions they have in the different ontogenetic stages during the development of venomous animals. Mass spectrometry imaging combined with spatial transcriptomics, in situ hybridization techniques, and modern computer tomography gives us further insights into the spatial distribution of toxins in the venom system and the function of the venom apparatus. All these evolutionary and biological insights contribute to more efficiently identify venom compounds, which can then be synthesized or produced in adapted expression systems to test their bioactivity. Finally, we critically discuss recent agrochemical, pharmaceutical, therapeutic, and diagnostic (so-called translational) aspects of venoms from which humans benefit.
Details
- Title
- Modern venomics-Current insights, novel methods, and future perspectives in biological and applied animal venom research
- Creators
- Bjoern M von Reumont (Corresponding Author) - Justus Liebig University Giessen, Institute for Insectbiotechnology, Heinrich Buff Ring 26-32, 35396 Giessen, GermanyGregor Anderluh - National Institute of ChemistryAgostinho Antunes - Universidade do PortoNaira Ayvazyan - Orbeli Institute of Physiology of NAS RA, Orbeli ave. 22, 0028 Yerevan, ArmeniaDimitris Beis - Developmental Biology, Centre for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation Academy of Athens, Athens 11527, GreeceFigen Caliskan - Eskişehir Osmangazi UniversityAna Crnković - National Institute of ChemistryMaik Damm - Technische Universität BerlinSebastien Dutertre - University of MontpellierLars Ellgaard - Department of Biology, University of Copenhagen, DK-2200 Copenhagen, DenmarkGoran Gajski - Institute for Medical Research and Occupational Health, Mutagenesis Unit, Ksaverska cesta 2, 10000 Zagreb, CroatiaHannah German - Amsterdam Institute of Molecular and Life Sciences, Division of BioAnalytical Chemistry, Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081HV Amsterdam, The NetherlandsBeata Halassy - University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Trg Republike Hrvatske 14, 10000 Zagreb, CroatiaBenjamin-Florian Hempel - BIH Center for Regenerative Therapies BCRT, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyTim Hucho - Translational Pain Research, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, GermanyNasit Igci - Nevşehir Hacı Bektaş Veli UniversityMaria P Ikonomopoulou - The University of Queensland, St Lucia, QLD 4072, AustraliaIzhar Karbat - 972WIS_INST___112Maria I Klapa - FORTH Institute of Chemical Engineering SciencesIvan Koludarov - Justus Liebig University Giessen, Institute for Insectbiotechnology, Heinrich Buff Ring 26-32, 35396 Giessen, GermanyJeroen Kool - Amsterdam Institute of Molecular and Life Sciences, Division of BioAnalytical Chemistry, Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081HV Amsterdam, The NetherlandsTim Lüddecke - Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, 35392 Gießen, GermanyRiadh Ben Mansour - University of GafsaMaria Vittoria Modica - Dept. of Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn, Via Po 25c, I-00198 Roma, ItalyYehu Moran - Department of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, IsraelAyse Nalbantsoy - Ege UniversityMaría Eugenia Pachón Ibáñez - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, SpainAlexios Panagiotopoulos - FORTH Institute of Chemical Engineering SciencesEitan Reuveny - 972WIS_INST___112Javier Sánchez Céspedes - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, SpainAndy Sombke - Department of Evolutionary Biology, University of Vienna, Djerassiplatz 1, 1030 Vienna, AustriaJoachim M Surm - Department of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, Faculty of Science, The Hebrew University of Jerusalem, Jerusalem 9190401, IsraelEivind A B Undheim - University of Oslo, Centre for Ecological and Evolutionary Synthesis, Postboks 1066 Blindern 0316 Oslo, NorwayAida Verdes - Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, José Gutiérrez Abascal 2, 28006 Madrid, SpainGiulia Zancolli - Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland
- Resource Type
- Journal article
- Publication Details
- Gigascience, Vol.11, giac048; 18/May/2022
- Language
- English
- DOI
- https://doi.org/10.1093/gigascience/giac048
- PMID
- 35640874
- Grant note
- CA19144 / European Cooperation in Science and Technology 271522021 / Deutsche Forschungsgemeinschaft We thank Ronald Jenner and Stuart Ainsworth for commenting and editing the final manuscript version. M.D. is grateful to Prof. R. D. Süßmuth for the supervision and support during the Ph.D. time, in which this manuscript was achieved. B.M.v.R. and I.K. thank Andreas Vilcinskas for support and work space at the Institute of Insectbiotechnology within the group Animal Venomics. B.M.v.R. finally thanks Ingo Ebersberger for his help and support. This work is funded by the European Cooperation in Science and Technology (COST, www.cost.eu) and based upon work from the COST Action CA19144 – European Venom Network (EUVEN, see https://euven-network.eu/). This review is an outcome of EUVEN Working Group 2 (“Best practices and innovative tools in venomics”) led by B.M.v.R. As coordinator of the group Animal Venomics until end 2021 at the Institute for Insectbiotechnology, JLU Giessen, B.M.v.R. acknowledges the Centre for Translational Biodiversity Genomics (LOEWE-TBG) in the programme “LOEWE – Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse's Ministry of Higher Education, Research, and the Arts. B.M.v.R. and I.K. further acknowledge funding on venom research by the German Science Foundation to B.M.v.R. (DFG RE3454/6-1). A.C., A.V., and G.Z. were supported by the European Union's Horizon 2020 Research and Innovation program through Marie Sklodowska-Curie Individual Fellowships (grant agreements No. A.C.: 896849, A.V.: 841576, and G.Z.: 845674). M.P.I. is supported by the TALENTO Program by the Regional Madrid Government (2018-T1/BIO-11262). T.H.'s venom research is funded by the DFG projects 271522021 and 413120531. L.E. was supported by grant No. 7017-00288 from the Danish Council for Independent Research (Technology and Production Sciences). N.I. acknowledges funding on venom research by the Research Fund of Nevsehir Haci Bektas Veli University (project Nos. ABAP20F28, BAP18F26). M.I.K. and A.P. acknowledge support from GSRT National Research Infrastructure structural funding project INSPIRED (MIS 5002550). G.A. acknowledges support from the Slovenian Research Agency grants P1-0391, J4-8225, and J4-2547. G.G. acknowledges support from the Institute for Medical Research and Occupational Health, Zagreb, Croatia. E.A.B.U. is supported by a Norwegian Research Council FRIPRO-YRT Fellowship No. 287462. Authors' Contributions Lead, major conceptualization, first draft, graphics, and illustration by B.M.v.R.; all authors wrote the main text and edited the final manuscript. Except for the first author, authors are listed alphabetically with respect to the last name. All authors have read and agreed to the published version of the manuscript.
- Record Identifier
- 993368184303596
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