Journal article
LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis
EMBO Journal, Vol.29(11), pp.1792-1802
Jun/2010
Abstract
Autophagy, a critical process for bulk degradation of proteins and organelles, requires conjugation of Atg8 proteins to phosphatidylethanolamine on the autophagic membrane. At least eight different Atg8 orthologs belonging to two subfamilies (LC3 and GATE-16/GABARAP) occur in mammalian cells, but their individual roles and modes of action are largely unknown. In this study, we dissect the activity of each subfamily and show that both are indispensable for the autophagic process in mammalian cells. We further show that both subfamilies act differently at early stages of autophagosome biogenesis. Accordingly, our results indicate that LC3s are involved in elongation of the phagophore membrane whereas the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. The EMBO Journal (2010) 29, 1792-1802. doi:10.1038/emboj.2010.74; Published online 23 April 2010
Details
- Title
- LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis
- Creators
- Hilla Weidberg (null) - 972WIS_INST___112Elena Shvets (null) - 972WIS_INST___112Tomer Shpilka (null) - 972WIS_INST___112Frida Shimron (null) - The Weizmann Institute of ScienceVera Shinder (null) - 972WIS_INST___100Zvulun Elazar (null) - 972WIS_INST___112
- Resource Type
- Journal article
- Publication Details
- EMBO Journal, Vol.29(11), pp.1792-1802; Jun/2010
- Number of pages
- 11
- Language
- English
- DOI
- https://doi.org/10.1038/emboj.2010.74
- Grant note
- Israel Science Foundation; Israeli Cancer Research Foundation; Weizmann Institute Minerva CenterWe thank Noboru Mizushima for the anti- Atg16 antibodies. We also thank Y Avivi for discussions and critical evaluation of the manuscript, and V Kiss for technical assistance. ZE is an incumbent of the Harold Korda Chair of Biology. This work was supported in part by the Israel Science Foundation, the Israeli Cancer Research Foundation, and the Weizmann Institute Minerva Center. The electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at The Weizmann Institute of Science._ALMAME_DELIMITER_
- Record Identifier
- 993266031003596
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