Journal article
Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex
Current Opinion in Structural Biology, Vol.23(5), pp.686-694
Oct/2013
Abstract
Cellulosomes are multi-enzyme complexes produced by anaerobic bacteria for the efficient deconstruction of plant cell wall polysaccharides. The assembly of enzymatic subunits onto a central non-catalytic scaffoldin subunit is mediated by a highly specific interaction between the enzyme-bearing dockerin modules and the resident cohesin modules of the scaffoldin, which affords their catalytic activities to work synergistically. The scaffoldin also imparts substrate-binding and bacterial-anchoring properties, the latter of which involves a second cohesin-dockerin interaction. Recent structure-function studies reveal an ever-growing array of unique and increasingly complex cohesin-dockerin complexes and cellulosomal enzymes with novel activities. A 'build' approach involving multimodular cellulosomal segments has provided a structural model of an organized yet conformationally dynamic supramolecular assembly with the potential to form higher order structures.
Details
- Title
- Insights into cellulosome assembly and dynamics; from dissection to reconstruction of the supramolecular enzyme complex
- Creators
- Steven P. Smith (null)Ed Bayer (null) - 972WIS_INST___112
- Resource Type
- Journal article
- Publication Details
- Current Opinion in Structural Biology, Vol.23(5), pp.686-694; Oct/2013
- Number of pages
- 9
- Language
- English
- DOI
- https://doi.org/10.1016/j.sbi.2013.09.002
- Grant note
- National Science and Engineering Research Council of Canada [RGPIN 398508]; Israel Science Foundation [1349/13]This work was supported by a National Science and Engineering Research Council of Canada discovery grant to SPS (RGPIN 398508) and by an Israel Science Foundation grant to EAB (No. 1349/13). E.A.B. is the incumbent of the Maynard I. and Elaine Wishner Chair of Bio-organic Chemistry._ALMAME_DELIMITER_
- Record Identifier
- 993266321003596
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