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Journal article
Evidence for new enantiospecific interaction force in chiral biomolecules
Chem, Vol.7(10), pp.2787-2799
14/Oct/2021
Abstract
Enantiospecific biorecognition interactions are key to many biological events. Commonly, bio-affinity values, measured in these processes, are higher than those calculated by available methods. We report here the first direct measurement of the interaction force between two chiral peptides (right- and left-handed helical polyalanine peptides) and the quantification of difference in the interaction force between homochiral and heterochiral pairs of molecules using atomic force microscope (AFM), together with supportive calculations based on a simple theoretical model. A force difference of 70 pN between same and opposite enantiomer interactions is measured. Additional measurements show spin dependency and fast decay of the interaction term, consistent with spin exchange interactions. This short range enantiospecific interaction term is especially relevant in crowded biological systems. The results shed light on the importance of spin and exchange interactions in biological processes.
Details
- Title
- Evidence for new enantiospecific interaction force in chiral biomolecules
- Creators
- Yael Kapon - Department of Applied Physics, Hebrew University, Jerusalem 91904, IsraelAbhijit Saha - Institute of Chemistry, the Hebrew University, Jerusalem 91904, IsraelTal Duanis-Assaf - Institute of Chemistry, the Hebrew University, Jerusalem 91904, IsraelThijs Stuyver - Institute of Chemistry, the Hebrew University, Jerusalem 91904, IsraelAmir Ziv - Department of Applied Physics, Hebrew University, Jerusalem 91904, IsraelTzuriel Metzger - Department of Applied Physics, Hebrew University, Jerusalem 91904, IsraelShira Yochelis - Hebrew University of JerusalemSason Shaik (Corresponding Author) - Hebrew University of JerusalemRon Naaman (Corresponding Author) - 972WIS_INST___136Meital Reches (Corresponding Author) - Institute of Chemistry, the Hebrew University, Jerusalem 91904, IsraelYossi Paltiel (Corresponding Author) - Hebrew University of Jerusalem
- Resource Type
- Journal article
- Publication Details
- Chem, Vol.7(10), pp.2787-2799; 14/Oct/2021
- Number of pages
- 14
- Publisher
- Elsevier Inc
- Language
- English
- DOI
- https://doi.org/10.1016/j.chempr.2021.08.002
- Grant note
- Y.P. and R.N. acknowledge the support of the John Templeton Foundation and the MOS Israel. R.N. acknowledges the partial support of the ISF and of the Minerva Foundation. S.S. and T.S. are supported by the ISF grant 520/18. T.S. acknowledges the Research Foundation-Flanders (FWO) for a position as a postdoctoral research fellow (1203419N). A.S. acknowledges the support of the Shunbrun Fellowship. Author contributions Y.K. and A.S. performed and analyzed the experimental results. T.D.-A. and A.Z. helped in the AFM measurements. T.M. and S.Y. helped in all the chemical aspects of the paper. T.S. did the modeling and theoretical calculations. S.S. supervised the theoretical part of the manuscript. S.Y., M.R., R.N., and Y.P. conceived the experimental part of the work and participated in planning and analyzing the results. All authors participated in writing the manuscript. All the data are presented in the manuscript and supplemental information.
- Record Identifier
- 993323815503596
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