Journal article
Cell-cycle progress in obligate predatory bacteria is dependent upon sequential sensing of prey recognition and prey quality cues
Proceedings of the National Academy of Sciences of the United States of America, Vol.112(44), pp.E6028-E6037
03/Nov/2015
Abstract
Predators feed on prey to acquire the nutrients necessary to sustain their survival, growth, and replication. In Bdellovibrio bacteriovorus, an obligate predator of Gram-negative bacteria, cell growth and replication are tied to a shift from a motile, free-living phase of search and attack to a sessile, intracellular phase of growth and replication during which a single prey cell is consumed. Engagement and sustenance of growth are achieved through the sensing of two unidentified prey-derived cues. We developed a novel ex vivo cultivation system for B. bacteriovorus composed of prey ghost cells that are recognized and invaded by the predator. By manipulating their content, we demonstrated that an early cue is located in the prey envelope and a late cue is found within the prey soluble fraction. These spatially and temporally separated cues elicit discrete and combinatory regulatory effects on gene transcription. Together, they delimit a poorly characterized transitory phase between the attack phase and the growth phase, during which the bdelloplast (the invaded prey cell) is constructed. This transitory phase constitutes a checkpoint in which the late cue presumably acts as a determinant of the prey's nutritional value before the predator commits. These regulatory adaptations to a unique bacterial lifestyle have not been reported previously.
Details
- Title
- Cell-cycle progress in obligate predatory bacteria is dependent upon sequential sensing of prey recognition and prey quality cues
- Creators
- Or Rotem (null)Zohar Pasternak (null)Eyal Shimoni (null) - 972WIS_INST___100Eduard Belausov (null)Ziv Porat (null) - 972WIS_INST___113Shmuel Pietrokovski (null) - 972WIS_INST___111Edouard Jurkevitch (null)
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences of the United States of America, Vol.112(44), pp.E6028-E6037; 03/Nov/2015
- Number of pages
- 10
- Language
- English
- DOI
- https://doi.org/10.1073/pnas.1515749112
- Grant note
- This research was support by Israel Science Foundation Grants 1583/12 and 1290/08 and German-Israeli Foundation for Scientific Research Grant I-1217-342.13/2012.
- Record Identifier
- 993262815103596
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