Journal article
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Science, Vol.365(6454), pp.699-704
16/Aug/2019
Abstract
Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, called Voltron, that uses bright and photostable synthetic dyes instead of protein-based fluorophores, thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In the mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously over a 15-minute period of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior.
Details
- Title
- Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
- Creators
- Ahmed S. Abdelfattah (null) - Janelia Research CampusTakashi Kawashima (null) - 972WIS_INST___123Amrita Singh (null) - Janelia Research CampusOndrej Novak (null) - Janelia Research CampusHui Liu (null) - Janelia Research CampusYichun Shuai (null) - Janelia Research CampusYi-Chieh Huang (null) - National Yang Ming UniversityLuke Campagnola (null) - Allen Institute for Brain ScienceStephanie C. Seeman (null) - Allen Institute for Brain ScienceJianing Yu (null) - Janelia Research CampusJihong Zheng (null) - Janelia Research CampusJonathan B. Grimm (null) - Janelia Research CampusRonak Patel (null) - Janelia Research CampusJohannes Friedrich (null) - Columbia UniversityBrett D. Mensh (null) - Janelia Research CampusLiam Paninski (null) - Columbia UniversityJohn J. Macklin (null) - Janelia Research CampusGabe J. Murphy (null) - Allen Institute for Brain ScienceKaspar Podgorski (null) - Janelia Research CampusBei-Jung Lin (null) - National Yang Ming UniversityTsai-Wen Chen (null) - National Yang Ming UniversityGlenn C. Turner (null) - Janelia Research CampusZhe Liu (null) - Janelia Research CampusMinoru Koyama (null) - Janelia Research CampusKarel Svoboda (null) - Janelia Research CampusMisha B. Ahrens (null) - Janelia Research CampusLuke D. Lavis (null) - Janelia Research CampusEric R. Schreiter (null) - Janelia Research Campus
- Resource Type
- Journal article
- Publication Details
- Science, Vol.365(6454), pp.699-704; 16/Aug/2019
- Number of pages
- 6
- Language
- English
- DOI
- https://doi.org/10.1126/science.aav6416
- Grant note
- We thank the Vivarium, Cell Culture, Instrument Design and Fabrication, Imaging, Molecular Biology, and Virus Production facilities at Janelia for assistance. Specifically, we thank B. Shields, D. Walpita, J. Cox, C. McGlynn, D. Alcor, A. Taylor, J. Rouchard, K. Ritola, X. Zhang, and J. Towne. We thank Z. Wei for discussions on data analysis. Funding: Supported by HHMI (A.S.A., T.K., A.S., O.N., H.L., Y.S., J.Y., J.Z., J.B.G., R.P., B.D.M., J.J.M., K.P., G.C.T., Z.L., M.K., K.S., M.B.A., L.D.L., and E.R.S.), Simons Collaboration on the Global Brain research awards 325171 and 542943SPI (M.B.A. and L.P.), IARPA MICRONS D16PC00003 (L.P.), NIH R01EB22913 (L.P.), Taiwan Ministry of Science and Technology MOST106-2628-B-010-004, MOST105-2628-B-010-005, MOST106-2320-B-010-012 and Taiwan National Health Research Institute NHRI-ex-107-10509NC (T.-W.C. and B.-J.L.), and the Allen Institute for Brain Science (L.C., S.C.S., and G.J.M.).
- Record Identifier
- 993263070403596
Metrics
13 Record Views