Journal article
Biocompatibility of Tungsten Disulfide Inorganic Nanotubes and Fullerene-Like Nanoparticles with Salivary Gland Cells
Tissue Engineering Part A, Vol.21(5-6), pp.1013-1023
Mar/2015
Abstract
Impaired salivary gland (SG) function leading to oral diseases is relatively common with no adequate solution. Previously, tissue engineering of SG had been proposed to overcome this morbidity, however, not yet clinically available. Multiwall inorganic (tungsten disulfide [WS2]) nanotubes (INT-WS2) and fullerene-like nanoparticles (IF-WS2) have many potential medical applications. A yet unexplored venue application is their interaction with SG, and therefore, our aim was to test the biocompatibility of INT/IF-WS2 with the A5 and rat submandibular cells (RSC) SG cells. The cells were cultured and subjected after 1 day to different concentrations of INT-WS2 and were compared to control groups. Growth curves, trypan blue viability test, and carboxyfluorescein succinimidyl ester (CFSE) proliferation assay were obtained. Furthermore, cells morphology and interaction with the nanoparticles were observed by light microscopy, scanning electron microscopy and transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy. The results showed no significant differences in growth curves, proliferation kinetics, and viability between the groups compared. Moreover, no alterations were observed in the cell morphology. Interestingly, TEM images indicated that the nanoparticles are uptaken by the cells and accumulate in cytoplasmic vesicles. These results suggest promising future medical applications for these nanoparticles.
Details
- Title
- Biocompatibility of Tungsten Disulfide Inorganic Nanotubes and Fullerene-Like Nanoparticles with Salivary Gland Cells
- Creators
- Elisheva B. Goldman (null)Alla Zak (null)Reshef Tenne (null) - The Weizmann Institute of ScienceElena Kartvelishvily (null) - 972WIS_INST___100Smadar Levin-Zaidman (null) - The Weizmann Institute of ScienceYoav Neumann (null)Raluca Stiubea-Cohen (null)Aaron Palmon (null)Avi-Hai Hovav (null)Doron J. Aframian (null)
- Resource Type
- Journal article
- Publication Details
- Tissue Engineering Part A, Vol.21(5-6), pp.1013-1023; Mar/2015
- Number of pages
- 11
- Language
- English
- DOI
- https://doi.org/10.1089/ten.tea.2014.0163
- Grant note
- ERC through MEDIF-2 PoC project; KAMIN project of the Office of Chief Scientist, Israeli Ministry of EconomyThe electron microscopy studies were conducted at the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging at the Weizmann Institute of Science. We are grateful to Dr. I. Sabanay and Dr. V. Shinder for TEM consulting and to Dr. S.R. Cohen for critical review of the article. R.T. acknowledges the support of the ERC support through MEDIF-2 PoC project and the KAMIN project of the Office of Chief Scientist, Israeli Ministry of Economy._ALMAME_DELIMITER_
- Record Identifier
- 993267818903596
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