Electron cryo-tomography using the scanning transmission modality (STEM)enables 3D reconstruction of unstained, vitrified specimens as thick as 1 μm or more. Contrast is related to mass/thickness and atomic number, providing quantifiable chemical characterization and mass mapping of intact prokaryotic and eukaryotic cells. Energy dispersive X-ray spectroscopy by STEM provides a simple, on-the-spot chemical identification of the elemental composition in sub-cellular organic bodies or mineral deposits. This chapter provides basic background and practical information for performing cryo-STEM tomography on vitrified biological cells.
Book chapter
CryoSTEM tomography in biology
Three-Dimensional Electron Microscopy, pp.197-215
2019
Abstract
Details
- Title
- CryoSTEM tomography in biology
- Creators
- Sharon G. Wolf (Corresponding Author) - 972WIS_INST___100Michael Elbaum (null) - 972WIS_INST___136
- Resource Type
- Book chapter
- Publication Details
- Three-Dimensional Electron Microscopy, pp.197-215; 2019
- Chapter Number
- Book Chapter: 10
- Number of pages
- 19
- Book Editors
- Müller-Reichert, Thomas ; Pigino, Gaia
- Series
- Methods in Cell Biology
- Publisher
- Academic Press Inc.
- Language
- English
- DOI
- https://doi.org/10.1016/bs.mcb.2019.04.001
- Grant note
- We thank Dr. Lothar Houben and Prof. Peter Rez for helpful discussions, and Drs. Anna Carlsson and Maarten Wirix from Thermo Fisher Scientific for discussions and guidance on our new systems. Our work on STEM development has been supported by the Israel Science Foundation.
- ISBN
- 9780128170182
- Record Identifier
- 993266792203596
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